Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Il Kyu Cho
Tanzania
Research Article
Identification and Classification of Rhizobia by Matrix-Assisted Laser
Desorption/Ionization Time-Of-Flight Mass Spectrometry
Author(s): Rui Zong Jia, Rong Juan Zhang, Qing Wei, Wen Feng Chen, Il Kyu Cho, Wen Xin Chen and Qing X LiRui Zong Jia, Rong Juan Zhang, Qing Wei, Wen Feng Chen, Il Kyu Cho, Wen Xin Chen and Qing X Li
Mass spectrometry (MS) has been widely used for specific, sensitive and rapid analysis of proteins and has shown a high potential for bacterial identification and characterization. Type strains of four species of rhizobia and Escherichia coli DH5α were employed as reference bacteria to optimize various parameters for identification and classification of species of rhizobia by matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF MS). The parameters optimized included culture medium states (liquid or solid), bacterial growth phases, colony storage temperature and duration, and protein data processing to enhance the bacterial identification resolution, accuracy and reliability. The medium state had little effects on the mass spectra of protein profiles. A suitable sampling time was between the exponential phase and the stationary phase. Consistent protein mass sp.. View More»
DOI:
10.4172/jpb.1000357