Journal of Cell Science & Therapy
Open Access

Jakub Grzesiak

Publications

• Research Article
  The Autologous Gelsolin Combined with Exogenous Nucleotides enhance Chondrogenic Differentiation in Equine Adipose Derived Mesenchymal Stromal Cells - An In Vitro Research
  Author(s): Krzysztof Marycz, Monika Maredziak, Agnieszka Smieszek, Jakub Grzesiak and Anna SiudzinskaKrzysztof Marycz, Monika Maredziak, Agnieszka Smieszek, Jakub Grzesiak and Anna Siudzinska
  
  Background: Nowadays, veterinary practice, involves treatment of disorders associated with cartilage degeneration using advanced therapy, based on mesenchymal stromal cells application. Preparation of cells for the transplantation requires in vitro culture and evaluation of their chondrogenic potential. Purpose: To determine if autologous gelsolin derived from equine serum and exogenous nucleotides may enhance the metabolic activity and chondrogenic differentiation of equine adipose derived mesenchymal stromal cells (EqASCs). Methods: Mesenchymal stem cells were isolated from equine subcutaneous fat tissue. Standard growth medium was supplemented with 1% of gelsolin or/and 0.1 mg/ml of nucleotides. Proliferation activity of the cells was determined basing on results obtained with cytotoxic assay and analysis of microve.. View More»
  DOI: 10.4172/2157-7013.1000174

  Abstract PDF

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