Journal of Clinical and Cellular Immunology
Open Access

James W. Ironside

Publications

• Research Article
  Comparative Study of Prions in Iatrogenic and Sporadic Creutzfeldt-Jakob Disease
  
  Author(s): Xiangzhu Xiao, Jue Yuan, Liuting Qing, Ignazio Cali, Jacqueline Mikol, Marie-Bernadette Delisle, Emmanuelle Uro-Coste, Liang Zeng, Mai Abouelsaad, Dimitris Gazgalis, Manuel Camacho Martinez, Gong-Xian Wang, Paul Brown, James W. Ironside, Pierluigi Gambetti, Qingzhong Kong and Wen-Quan ZouXiangzhu Xiao, Jue Yuan, Liuting Qing, Ignazio Cali, Jacqueline Mikol, Marie-Bernadette Delisle, Emmanuelle Uro-Coste, Liang Zeng, Mai Abouelsaad, Dimitris Gazgalis, Manuel Camacho Martinez, Gong-Xian Wang, Paul Brown, James W. Ironside, Pierluigi Gambetti, Qingzhong Kong and Wen-Quan Zou
  
  Differentiating iatrogenic Creutzfeldt-Jakob disease (iCJD) from sporadic CJD (sCJD) would be useful for the identification and prevention of human-to-human prion transmission. Currently, the diagnosis of iCJD depends on identification of a recognized source of contamination to which patients have been exposed, in addition to fulfilling basic requirements for the establishment of diagnosis of CJD. Attempts to identify differences in clinical manifestations, neuropathological changes and pathological prion protein (PrPSc) between iCJD and sCJD have been unsuccessful. In the present study, using a variety of more sophisticated methods including sucrose step gradient sedimentation, conformational stability immunoassay, protein misfolding cyclic amplification (PMCA), fragment-mapping, and transmission study, we show no significant differences in gel profiles, oligomeric state, .. View More»
  DOI: 10.4172/2155-9899.1000240

  Abstract

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