Anatomy & Physiology: Current Research
Open Access

Jan van der Greef

Publications

• Research Article
  The Relation between Non-adipose Muscle Fat and Hepatic Steatosis Studied with Localized 1H Magnetic Resonance Spectroscopy (1H MRS) and LC-MS Techniques
  Author(s): van Ginneken VJT, Ronald Booms, Elwin Verheij, Evert de Vries and Jan van der Greef van Ginneken VJT, Ronald Booms, Elwin Verheij, Evert de Vries and Jan van der Greef
  
  Aim/objective: In this study we investigated ectopic fat storage in the muscle and the liver using 1H Magnetic Resonance Spectroscopy (1H-MRS). The inability to store fat in adipose tissue leads to ectopic Triacylglycerol (TG) accumulation in muscle followed by the liver: the so called “overflow hypothesis”. It is assumed that when steatosis occurs in organs like the liver we can speak from “Metabolic Syndrome”. Methods: We compared the effects of two different diet interventions, 24 h-starvation and 40 days High-fat diet (+0.25% cholesterol and 45% energy from bovine lard) with control mice. Characterization of lipid molecular species in non-adipose muscle homogenate was performed by comparing the groups using liquid chromatography coupled to mass spectrometry (LC-MS) techniques following a Systems Biology lipidomics.. View More»
  DOI: 10.4172/2161-0940.1000245

  Abstract PDF

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