Journal of Proteomics & Bioinformatics
Open Access

Je-Yoel Cho

Publications

• Research Article
  Clinically Applicable Diagnostics Assay Development for Lung Cancer Biomarker, SAA
  Author(s): Hye-Jin Sung, Sang-Su Na, Keyung-Jo Seul, Hyo-Sung Jeon, Jae Yong Park and Je-Yoel ChoHye-Jin Sung, Sang-Su Na, Keyung-Jo Seul, Hyo-Sung Jeon, Jae Yong Park and Je-Yoel Cho
  
  Background: Serum Amyloid A (SAA) is an acute phase protein and has been reported as a lung cancer biomarker. Many cancer protein biomarkers have been reported but few are currently in clinical application. This is due to the obstacles in the process from biomarker discovery to biomarker application: validation in large scale clinical samples and paired antibody production for the development of immunoassay-based diagnostics. Methods: To develop immunoassay-based diagnostics of SAA, we produced anti-SAA monoclonal antibodies after bacterial production of pure SAA whole protein immunogen. Using two SAA specific monoclonal antibody clones, we developed two types of diagnostics; ELISA and rapid tester diagnostics. Hundreds of clinical samples were tested using the immunoassay diagnostics developed. Results: The diagnostic or differential diagnostic ability of lung cancer .. View More»
  DOI: 10.4172/jpb.1000333

  Abstract PDF

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