Pediatrics & Therapeutics
Open Access

Jie Deng

Publications

• Research Article
  Development of Two Multiplex Real-Time PCR Assays for the Rapid Detection of RNA and DNA Viruses Associated with Gastroenteritis in Pediatric Patients
  Author(s): Dongmei Chen,Yu Sun,Linqing Zhao*,Yuan Qian,Runan Zhu,Liying Liu,Liping Jia,Huijin Dong,Jie Deng,Li DengDongmei Chen,Yu Sun,Linqing Zhao*,Yuan Qian,Runan Zhu,Liying Liu,Liping Jia,Huijin Dong,Jie Deng,Li Deng
  
  Background: Viral gastroenteritis is one of the most common causes of morbidity and mortality in infants and young children in China. Objectives: To develop a rapid and sensitive real time PCR method capable of detecting several viruses associated with gastroenteritis in pediatric patients simultaneously. Methods: Two multiplex real-time PCR assays, the first targeting the RNA viruses: rotavirus, norovirus and parachovirus and the second the DNA viruses: human adenovirus and human bocavirus 2, were designed and assessed for their specificity and sensitivity. The multiplex assays were evaluated using clinical samples and compared to conventional RT-PCR/PCR. Results The multiplex assays developed in the study were successful in detecting the five target viruses. No cross-reactions with a panel of other human viruses were presented. The assays were sensitive enough to detect as little as .. View More»
  DOI: 10.4172/2161-0665.1000208

  Abstract PDF

Relevant Topics