Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Jinfeng Ding
Indiana
Research Article
Antitumor Activity of Polysaccharides and Saponin Extracted from Sea Cucumber
Author(s): Xiurong Su, Caiyun Xu, Yanyan Li, Xiang Gao, Yanru Lou and Jinfeng DingXiurong Su, Caiyun Xu, Yanyan Li, Xiang Gao, Yanru Lou and Jinfeng Ding
The density gradient of S180 cells were treated with various extracts at a concentration of polysaccharides (P1 and P2) and saponins (S1 and S2) measured after 22h, 44h, 68h and 92 h post-treatment. P1, P2, and saponin S1 were extracted from Acaudina leucoproct, and S2 was extracted from the concentrated liquid of Stichopus japonica. The cytotoxic effects of S1 and S2 on S180 cells was demons treated dose and time dependent, using MTT assay. Saponin S2 exhibited the highest potency against S180 cells, with an IC50 of 41.04 µg/ml at 44 h. Additionally, by Annexin V/PI staining, we observed more viable cells under polysaccharides treatment compared to the saponins. These results indicate that the in vitro anti-tumor effects of saponins S1 and S2 are more potent than those of polysaccharides P1 and P2... View More»
DOI:
10.4172/2155-9899.1000105