Journal of Chromatography & Separation Techniques
Open Access

Jing Yang

Publications

• Research Article
  A Simple HPLC Method for the Separation of Colistimethate Sodium and Colistin Sulphate
  Author(s): Ligai Bai, Zhengyue Ma, Gengliang Yang, Jing Yang and Jia Cheng Ligai Bai, Zhengyue Ma, Gengliang Yang, Jing Yang and Jia Cheng
  
  In this paper, a simple high performance liquid chromatography (HPLC) method for the determination of colistimethate sodium in the productions of synthesis from colistin E sulphate was established. A HPLC gradient which was: 20% B+80% A changed to 50% B+50% A in 10 min (A was 0.05% TFA aqueous solution and B was acetonitrile) was used and the separation was realized in 8 minutes. Moreover, this method was also used in the separation of colistin sulphate with good resolution. Compared to the methods reported previously, the present method was finished easier in a much shorter time which is 6 min. LC-MS was used to detect colistin sulphate and the result showed that the two compositions were colistin sulphate E1 and E2 as expected. Good separation and reproducibility were obtained. .. View More»
  DOI: 10.4172/2157-7064.1000105

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