Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Jishang Gong
China
Research Article
Molecular Cloning and Bioinformatics Analysis of APEX1, a DNA Base
Excision Repair Enzyme from the Tianzhu White Yak (Bos grunniens)
Author(s): Quanwei Zhang, Xueying Wang, Jishang Gong, Youji Ma, Yong Zhang and Xingxu ZhaoQuanwei Zhang, Xueying Wang, Jishang Gong, Youji Ma, Yong Zhang and Xingxu Zhao
The molecular genetics of searching candidate gene on high-attitude adaptability and resistance to cold has become a worldwide hot topic in plateau biology, especial in human and yak. APEX1 as a reference gene, playing a crucial role in Base Excision Repair (BER) enzyme, researches about yak APEX1 can help to understand the adaption of yak in plateau, and then further to increase its economic value. Full length cDNA sequence of apurinic/ apyrimidinicsite endonuclease 1 (APEX1) was cloned from Tianzhu white yak to study the roles of APEX1 and its mechanisms in stress resistance. PCR and RACE techniques were used to obtain the full length of APEX1 cDNA sequence. The result indicated that the full length cDNA of yak APEX1 was 1391 bp (GenBank accession No. KF611690), with an ORF 957 bp encoding 318 amino acids. The amino sequence analysis indicated that APEX1 was highly conserved in most.. View More»
DOI:
10.4172/jpb.1000319