Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
JO Lay Jr.
Tanzania
Research Article
Identification and Structural Characterization of Avian Beta-Defensin 2 Peptides from Pheasant and Quail
Author(s): L Kannan, R Liyanage, JO Lay Jr., B Packialakshmi, NB Anthony and NC Rath
L Kannan, R Liyanage, JO Lay Jr., B Packialakshmi, NB Anthony and NC Rath
Pheasant and quail orthologs of Avian β-Defensin 2 (AvBD2) were identified by screening heterophil extracts using Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry (MS), comparative profiling, chemical, and bioinformatic characterizations. In heterophil extracts of each pheasant and quail, we observed a single high intensity mass peak, corresponding to m/z 4114.8 and 4163.8, respectively which upon reduction and alkylation were shifted by a mass difference of 348 Da indicative of the modification of 3 internal disulfide bonds that exist in these peptides. The unmodified peptides, m/z 4114 and 4163, and their Carbamidomethylated (CAM) derivatives were purified by reverse phase HPLC. Using the purified peptides we determined their partial sequences by trypsin digestion followed by MALDI-TOF-MS.. View More»
DOI:
10.4172/jpb.1000258