Journal of Chromatography & Separation Techniques
Open Access

Jody K. Takemoto

Publications

• Research Article
  An LC/MS Assay for Analysis of Vorinostat in Rat Serum and Urine: Application to a Pre-Clinical Pharmacokinetic Study
  Author(s): Elham A. Mohamed, Mahasen M. Meshali, Casey L. Sayre, Stephanie E. Martinez, Connie M. Remsberg, Jody K. Takemoto, Thanaa M. Borg, Abdel Monem M. Foda and Neal M. Davies Elham A. Mohamed, Mahasen M. Meshali, Casey L. Sayre, Stephanie E. Martinez, Connie M. Remsberg, Jody K. Takemoto, Thanaa M. Borg, Abdel Monem M. Foda and Neal M. Davies
  
  The utility of currently reported liquid chromatography mass spectrometry methods for quantitation of vorinostat in plasma or serum may be limited. These methods employ time consuming and expensive procedures such as solid phase extraction or limited-access techniques such as high turbulence liquid chromatography coupled with column switching. This study offers a simple isocratic LC/MS method validated for determination of vorinostat in both rat serum and urine. Proteins were precipitated from serum using ice-cold acetonitrile and daidzein was used as internal standard. Separation was achieved using a Phenomenex  Luna ®  C 18  (2) (5 μm, 250 x 4.60 mm) column and isocratic elution with a mobile phase consisting of acetonitrile, water, and formic acid (30:70:0.1, v/v/v). Mass spectrometry detection using electrospray positive-mode ionization with selected ion moni.. View More»
  DOI: 10.4172/2157-7064.1000118

  Abstract PDF

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