Mycobacterial Diseases
Open Access

Johnson Welishe

Publications

• Research Article
  Sero-diagnosis of Active Mycobacterium tuberculosis Disease among HIV Coinfected Persons using Thymidylate Kinase based Antigen and Antibody Capture Enzyme Immuno-Assays
  Author(s): Misaki Wayengera, Ivan Mwebaza, Johnson Welishe, Cynthia Nakimuli, David P Kateete, Eddie Wampande, Samuel Kirimunda, Lois Bayigga, Carol Musubika, Peace Babirye, Benon Asiimwe and Moses L Joloba Misaki Wayengera, Ivan Mwebaza, Johnson Welishe, Cynthia Nakimuli, David P Kateete, Eddie Wampande, Samuel Kirimunda, Lois Bayigga, Carol Musubika, Peace Babirye, Benon Asiimwe and Moses L Joloba
  
  Background: Clinical and laboratory diagnosis of Active Tuberculosis (ATB) and latent Mycobacterium Tuberculosis (M. tuberculosis) infections (LTBI) among people living with HIV/AIDS (PLWHA) presents formidable challenges. In the past, WHO issued an advisory against the use of existing TB sero-diagnostics? Emerging evidence, however, points to a precision of TB sero-diagnostics based on secretory rather than structural M. tuberculosis antigens. We hypothesized that secretory levels of M. tuberculosis thymidylate kinase (TMKmt) can designate ATBI from LTBI and no TB (NTB). Here, we report in-house validation studies of levels of TMKmt antigen (Ag) and host specific TMKmt antibody (Ab) amongst HIV +ve and HIV -ve participants. Methods and Results: Direct TMKmt Ag and host specific IgG Ab detection EIAs were conducted on broadly consented, stor.. View More»
  DOI: 10.4172/2161-1068.1000241

  Abstract PDF

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