Advances in dairy Research
Open Access
ISSN: 2329-888X
+44 1300 500008
ISSN: 2329-888X
+44 1300 500008
Juan J Loor
Urbana, IL 61801
Tanzania
Research Article
Transcriptomics Comparisons of Mac-T cells Versus Mammary Tissue during Late Pregnancy and Peak Lactation
Author(s): Afshin Hosseini, Rekha Sharma, Massimo Bionaz and Juan J LoorAfshin Hosseini, Rekha Sharma, Massimo Bionaz and Juan J Loor
Epithelial cell cultures, including immortalized cells, have been used extensively to help answer basic questions of mammary gland physiology under normal or diseased conditions. The usefulness of using cell culture depends on the degree of identify compared to the in vivo mammary epithelium. Our objective was to compare the transcriptome by means of microarray and qPCR of immortalized bovine mammary epithelial cells (Mac-T) cultivated in plastic (i.e., 2 dimensional system) vs. mammary tissue obtained during late-pregnancy (-30 DIM) and peak lactation (+60 DIM). Functional analysis of microarray data was performed using enrichment analysis tools and the Dynamic Impact Approach (DIA). Overall, between 37% (vs. +60 DIM) and 44% (vs. -30 DIM) of the measured annotated genes were deemed as differentially expressed (DEG) between Mac-T cells and mammary tissue. These data, together with th.. View More»
DOI:
10.4172/2329-888X.1000103