Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Jun Thorsteinsdottir
Germany
Research Article
Quantitative Analysis of Liposomal Heat Shock Protein 70 (Hsp70) in the Blood of Tumor Patients Using a Novel LipHsp70 ELISA
Author(s): Stephanie Breuninger, Janina Erl, Clemens Knape, Sophie Gunther, Ivonne Regel, Franz Rödel, Udo S Gaipl, Jun Thorsteinsdottir, Lydia Giannitrapani, Anne M Dickinson and Gabriele MulthoffStephanie Breuninger, Janina Erl, Clemens Knape, Sophie Gunther, Ivonne Regel, Franz Rödel, Udo S Gaipl, Jun Thorsteinsdottir, Lydia Giannitrapani, Anne M Dickinson and Gabriele Multhoff
Elevated levels of the stress-inducible heat shock protein 70 (Hsp70) in the peripheral circulation have been reported for many tumor entities. In line with these findings we have shown that Hsp70 membrane-positive tumor cells actively release Hsp70 in exosome-like lipid vesicles. Since most commercial Hsp70 Enzyme-Linked Immunosorbent Assays (ELISAs) are not validated for the detection of liposomal Hsp70 in serum, the lipHsp70 ELISA was established using the monoclonal antibody cmHsp70.1 as a detection reagent. This antibody has been reported to recognize free and membrane-bound Hsp70 on living tumor cells. Validation of the ELISA showed a high assay precision and linearity in a concentration range of 0.36-17.4 ng/ml. A comparison of the recovery of spiked Hsp70 in buffer and serum samples revealed a significantly better recovery using the lipHsp70 .. View More»
DOI:
10.4172/2155-9899.1000264