Rheumatology: Current Research
Open Access
ISSN: 2161-1149 (Printed)
+44-77-2385-9429
ISSN: 2161-1149 (Printed)
+44-77-2385-9429
Jürgen Mollenhauer
NMI Natural and Medical Sciences Institute at the University of Tubingen, Markwiesenstr. 55, 72770 Reutlingen,
Germany
Research Article
Quality Control for Mesenchymal Stromal Cells: Chondrogenesis as a Standard Condition?
Author(s): Jana Müller, Jürgen Mollenhauer, Rocky Tuan and Karin BenzJana Müller, Jürgen Mollenhauer, Rocky Tuan and Karin Benz
Objectives: The application of mesenchymal stromal precursor cells (MSC) in clinical settings requires a pharmaceutical definition of cell quality including identity, purity, and potency of the individual preparations. In vitro chondrogenesis is presented to define identity and potency independently of specific laboratory conditions.
Methods: MSC obtained from 34 patients undergoing hip replacement surgeries were expanded and brought to chondrogenic differentiation in two laboratories in the USA and in Germany. Cell culture protocols were modeled along established procedures with local variations (primary isolation, serum, hormones, micromass and hydrogel cultures). Chondrogenic differentiation in micromasses and in hydrogel encapsulated cells was analyzed by mRNA quantification, and by determination of glycosaminoglycan content.
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DOI:
10.4172/2161-1149.S3-003