Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access

Kafi M

Publications

• Research Article
  The In Vitro Effect of Adiponectin on Early Bovine Embryonic Development and Transcriptomic Markers of Oocyte Competence
  Author(s): Divar MR, Kafi M, Mohammadi A and Azari M Divar MR, Kafi M, Mohammadi A and Azari M
  
  Objectives: The current study aimed to investigate the in vitro effects of physiologic concentration of adiponectin in follicular fluid of pre-ovulatory bovine ovarian follicles on: 1) the bovine oocyte maturation and the early developmental rates of bovine embryos in vitro; 2) the expression profiles of some developmentally important marker genes in the bovine cumulus cells and oocytes. Materials and Methods: The cumulus-oocyte complexes (COCs) were aspirated from abattoir ovaries and cultured in vitro in the presence/absence of the recombinant human (rh) adiponectin (5 μg/mL) and used for real time RT-QPCR and in vitro embryo production assays. Results: Adiponection had no significant effects on nuclear maturation and cumulus expansion rates (p > 0.05). The expression levels of hyaluronic synthase 2 gene (H.. View More»
  DOI: 10.4172/2375-4508.1000172

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