Journal of Proteomics & Bioinformatics
Open Access

Karen L Leach

Publications

• Research Article
  Assessing Hepatoxicants based on High-throughput Quantitative SILAC Proteomics and Causal Biological Networks
  Author(s): Ahmed Enayetallah, Sashi Nadanaciva, Paul Ajuh, Cristina Vazquez Martin, Amy Wheat, Angus Lamond, Daniel Ziemek and Karen L Leach Ahmed Enayetallah, Sashi Nadanaciva, Paul Ajuh, Cristina Vazquez Martin, Amy Wheat, Angus Lamond, Daniel Ziemek and Karen L Leach
  
  Drug-induced liver injury can result in the termination of compounds in preclinical development, as well as withdrawal of marketed drugs. Identification of the signaling pathways and proteins involved in injury is an important step towards establishing assays that could be utilized to identify potential hepatotoxicants early in the drug discovery process. In this study we used high throughput quantitative mass spectrometry proteomics involving Stable Isotope Labeling with Amino acids in Cell culture (SILAC) leveraged by a recently developed systems biology approach (Causal Reasoning Engine, CRE) to investigate the effects of hepatotoxic compounds on the cellular proteome of HepG2 cells. Cells were treated with various concentrations of nefazadone, nimesulide, nomifensine, or glafenine, all of which cause hepatotoxicity in humans. Buspirone and rosiglitazone were used as comparator com.. View More»
  DOI: 10.4172/jpb.1000352

  Abstract PDF

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