Journal of Chromatography & Separation Techniques
Open Access

Katherine Lembright

Publications

• Research Article
  Quantification of Buprenorphine, Norbuprenorphine and 6-Monoacetylmorphine in Urine by Liquid Chromatography-Tandem Mass Spectrometry
  
  Author(s): Chao Yuan, Katherine Lembright, Courtney Heideloff and Sihe Wang Chao Yuan, Katherine Lembright, Courtney Heideloff and Sihe Wang
  
  Monitoring pain management medications and illicit drugs in urine is commonly used to assess patient compliance. Previously, we developed a liquid chromatography tandem mass spectrometry (LC-MS/MS) method to measure 19 analytes important for pain management. In the current report, we validated this method for two additional drugs, buprenorphine and heroin. For buprenorphine, we quantified both the parent drug and its major metabolite, norbuprenorphine. For heroin, we monitored its unique metabolite, 6-monoacetylmorphine (6-MAM). Urine samples were subjected to enzymatic hydrolysis prior to turbulent flow online extraction and LC-MS/MS analysis. No matrix effect or interference was found. Lower limits of quantifications were 9.7, 9.6, and 4.9 ng/mL for buprenorphine, norbuprenorphine and 6-MAM, respectively. Within the linear range, analytical recovery was 80.5-113.0% for all analytes... View More»
  DOI: 10.4172/2157-7064.1000174

  Abstract

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