Biochemistry & Pharmacology: Open Access
Open Access

Kathryn Leake

Publications

• Research Article
  Apoptosis and Differentiation of K562 Cells by Targeting GST-O1 to Inhibit 4-HNE Metabolism
  Author(s): Kathryn Leake, Jyotsana Singhal, Sharad S Singhal and Sanjay Awasthi Kathryn Leake, Jyotsana Singhal, Sharad S Singhal and Sanjay Awasthi
  
  Bcr-Abl kinase inhibitors are very effective drugs for treatment of chronic myelogenous leukemia (CML), but treatment options are limited and relatively ineffective for patients with de-novo or acquired resistance. The K562 human erythroleukemia cell line, derived from a pleural effusion during blast crisis of a CML patient, is very useful for studying hematopoietic differentiation because it undergoes differentiation and apoptosis in response to chemicals that propagate lipid-peroxidation. 4-hydroxynonenal (4-HNE), a reactive aldehyde produced from peroxidation of polyunsaturated fatty acids, is metabolized primarily by glutathione S-transferases (GSTs). 4-HNE causes differentiation, apoptosis and necrosis in K562 cells, but cannot be used as a drug for resistant CML because of its highly toxic nature. Present studies addressed the possibility of developing an alternative targeted tr.. View More»
  DOI: 10.4172/2167-0501.1000144

  Abstract PDF

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