ISSN: 2167-0412
+44 1300 500008
Kaul T
Nutritional Improvement of Crops Group - Plant Biology, International Centre for Genetic Engineering and Biotechnology,
Aruna Asaf Ali Marg, New Delhi-110 067
India
Research Article
Establishment of Efficient Regeneration System from Leaf Discs in Long Pepper an Important Medicinal Plant (Piper longum L.)
Author(s): Sathelly K, Podha S, Pandey S, Mangamuri U and Kaul T
Sathelly K, Podha S, Pandey S, Mangamuri U and Kaul T
Cultivation of Piper longum L. till recently was not very common; still it is extensively collected from the wild, threatening the very existence of the plant. Hence, it is alarming that there is an urgent need not only cultivate this plant but also develop conservation strategies. Conventional propagation is overwhelmed with problems of poor seed viability low percentage of germination, scanty and delayed rooting of vegetative cuttings indicating there is a need for alternative propagation methods. In vitro technique is an alternative approach to solve the problem. Therefore, the current research was aimed at developing a promising in vitro mass propagation protocol for Piper longum L. using leaf as explant. Murashige and Skoog medium was used throughout the experiment. For callus induction, MS medium supplemented with alone or in combination of IAA (1 to 2 mg/l) and BAP (1 to 2 mg/l.. View More»
DOI:
10.4172/2167-0412.1000248