Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Kazuyuki Nakamura
Japan
Research Article
Proteomic Differential Display Analysis Reveals Decreased Expression of
PEA-15 and Vimentin in FABP7-Deficient Astrocytes
Author(s): Majid Ebrahimi, Kazem Sharifi, Ariful Islam, Hirofumi Miyazaki, Yuki Yasumoto, Yoshiteru Kagawa, Yui Yamamoto, Takao Kitagawa, Yasuhiro Kuramitsu, Kazuyuki Nakamura and Yuji Owada
Majid Ebrahimi, Kazem Sharifi, Ariful Islam, Hirofumi Miyazaki, Yuki Yasumoto, Yoshiteru Kagawa, Yui Yamamoto, Takao Kitagawa, Yasuhiro Kuramitsu, Kazuyuki Nakamura and Yuji Owada
Fatty acid binding proteins (FABPs) are intracellular lipid chaperones which mediate the uptake, transportation and signaling roles of fatty acids. Former studies suggest that FABP7 regulates proliferation and differentiation of radial glia and astrocytes and is associated with various central nervous system diseases including glioma and neuropsychiatric diseases. However, the underlying mechanism is poorly understood. To further explore the mechanistic roles of FABP7 on astrocyte function, the proteome of astrocytes cultured from Fabp7 KO mice was compared with wild-type counterparts by two-dimensional gel electrophoresis (2-DE). We found that 16 protein spots showed differential expression intensity on 2-DE gels. Among them, seven spots appeared elevated, and nine spots appeared decreased in Fabp7 KO astrocytes. Selected spots were analyzed using liquid chromatography tandem mass sp.. View More»
DOI:
10.4172/jpb.1000347