Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Kersti V Klaamas
Estonia
Review Article
The Characterization of Cross-reactive Antibodies to Thomsen- Friedenreich a/b and Related Glycan-conjugates with Polyacrylamide Carriers in Patients with Gastrointestinal Cancer
Author(s): Eugeniy P Smorodin, Oleg A Kurtenkov, Boris L Sergeyev, Kersti V Klaamas and Jelena G IzotovaEugeniy P Smorodin, Oleg A Kurtenkov, Boris L Sergeyev, Kersti V Klaamas and Jelena G Izotova
The level of IgG and IgM antibodies (Abs) to the tumor-associated Thomsen-Friedenreich antigen (TF, Galβ1- 3GalNAcα) in the serum of patients with gastrointestinal cancer is reduced and the elevated anti-TF IgG level is positively associated with survival of patients with gastric cancer as shown earlier using ELISA with the TFpolyacrylamide (TF-pAA, an amide-type conjugate). The reactivity of Abs to the standard conjugate TF-PAA is low. To characterize the specificity of Abs, they were affinity-isolated from sera of patients by using different TF-sorbents. These were: 1) IgG populations that differed in the reactivity and cross-reactivity to TF, TFβ (Galβ1-3GalNAcβ), GA1 and Gb5tri (the Gb5 trisaccharide, Gal1-3GalNAcβ1-3Gal) conjugates. However, all the populations showed a crossreactivity to the pAA-carrier. 2) The pAA-carrier-independent cro.. View More»
DOI:
10.4172/2155-9899.S5-001