Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Kongsak Boonyapranai
Thailand
Research Article
Proteomic Analysis of Candidate Prognostic Urinary Marker for Cervical Cancer
Author(s): Paitoon Aobchey, Hataichanoke Niamsup, Sitthicha Siriaree, Boonyaras Sookkheo, Kongsak Boonyapranai and Shui-Tein ChenPaitoon Aobchey, Hataichanoke Niamsup, Sitthicha Siriaree, Boonyaras Sookkheo, Kongsak Boonyapranai and Shui-Tein Chen
Urinary proteins are an important component in urine samples that can be used as biological indicators for clinical diagnosis of diseases, such as proteinuria, kidney failure, diseases in the bladder, urinary tract and related organs. In this present work, we investigated candidate protein biomarkers in urine for diagnosis of cervical cancer using proteomic approach. The urine samples were concentrated and de-salted through ultrafiltration (3 kDa), followed by Spin-50 mini-sephadex column and were then separated by two-dimensional electrophoresis (2-DE) and annotated by mass spectrometer. Comparing 2-DE results between individual healthy donors and cervical cancer, patients showed that 4 urinary proteins; Protocadherin-8, Aryl hydrocarbon receptor nuclear translocatorlike protein 2, Serum albumin and Endorepellin, C-terminal domain V of perlecan were overexpressed by two-fold in the p.. View More»
DOI:
10.4172/jpb.1000287