Chemotherapy: Open Access
Open Access
ISSN: 2167-7700
ISSN: 2167-7700
Lei Yin
Indiana
Research Article
Down-Regulation of PER2 Increases Apoptosis of Gliomas after X-Ray
Irradiation
Author(s): Lei Yin, Wenbin Liu, Hechun Xia, Xiaoxiong Jia and David Leavesley
Lei Yin, Wenbin Liu, Hechun Xia, Xiaoxiong Jia and David Leavesley
Period2 (PER2), a core circadian gene, not only modulates circadian rhythm but also may play an important role in other biological processes including pathways involved in the proliferation and apoptosis of tumor cells. In this study, we investigated the mechanism by which downregulated expression of PER2 promotes apoptosis of wild-type TP53 human glioma U343 cells exposed to X-rays. U343 cells were irradiated with 6mV 10Gy X-ray irradiation after infection with shRNA lentivirus to reduce expression of PER2, and then analyzed by several methods such as SCGE analysis, flow cytometry, RT-PCR, and western blotting. Compared with controls, U343 cells expressing low levels of PER2 showed serious DNA damage when exposed to X-ray irradiation in SCGE analysis (P<0.05), and higher death rates in flow cytometry assay (P<0.05). RT-PCR and western blot analysis both revealed decreased expre.. View More»
DOI:
10.4172/2167-7700.1000228