Clinical & Experimental Cardiology
Open Access

Leonard L. Bailey

Publications

• Research Article
  Isolation, Characterization, and Spatial Distribution of Cardiac Progenitor Cells in the Sheep Heart
  Author(s): Xuwei Hou, Nancy Appleby, Tania Fuentes, Lawrence D. Longo, Leonard L. Bailey and Nahidh Hasaniya and Mary Kearns-JonkerXuwei Hou, Nancy Appleby, Tania Fuentes, Lawrence D. Longo, Leonard L. Bailey and Nahidh Hasaniya and Mary Kearns-Jonker
  
  Background: Laboratory large animal models are important for establishing the efficacy of stem cell therapies that may be translated into clinical use. The similarity of ovine and human cardiovascular systems provides an opportunity to use the sheep as a large animal model in which to optimize cell-based treatments for the heart. Recent clinical trials in humans using endogenous cardiovascular progenitor cells report significant improvement in cardiac function following stem cell-based therapy. To date, however, endogenous cardiovascular progenitor cells have not been isolated from the sheep heart. Methods: Cardiovascular cells expressing SSEA-4, CD105 and c-kit were isolated by flow cytometry and cloned from the right atrium of neonatal sheep. The expression of GATA-4, c-kit, and Isl1 was identified by PCR in the cloned cells. Immunohistochem.. View More»
  DOI: 10.4172/2155-9880.S6-004

  Abstract PDF

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