Journal of Clinical and Experimental Ophthalmology
Open Access

Liang-Jun Yan

Publications

• Research Article
  Two-Dimensional Differential In-Gel Electrophoresis (2D-DIGE) Reveals Proteins Associated with Cross-Linked Actin Networks in Human Trabecular Meshwork Cells
  Author(s): Jaclyn Y Bermudez, Hannah C Webber, Gaurang C Patel, Liang-Jun Yan, Abbot F Clark and Weiming Mao Jaclyn Y Bermudez, Hannah C Webber, Gaurang C Patel, Liang-Jun Yan, Abbot F Clark and Weiming Mao
  
  Background: The primary risk factor for primary-open angle glaucoma (POAG) is increased intraocular pressure (IOP). In POAG patients, the outflow resistance through the trabecular meshwork (TM) is abnormally elevated. One of the important glaucoma-associated pathological changes in the TM is formation of excessive cross-linked actin networks (CLANs). CLANs are web-like polygonal structures found in confluent glaucoma TM cells and tissues. Glaucoma-associated factors transforming forming growth factor beta 2 (TGFβ2) and glucocorticoids induce CLAN formation in non-glaucoma TM cells (NTM). CLANs may increase cell stiffness and alter homeostasis, and thereby contribute to elevated IOP. Methods: We used a proteomic approach to identify CLAN-associated proteins. We treated confluent primary NTM cells with 0.1% ethanol (EtOH;.. View More»
  DOI: 10.4172/2155-9570.1000584

  Abstract PDF

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