Journal of Clinical and Experimental Ophthalmology
Open Access

Lihua Kang

Publications

• Research Article
  Altered Expression and DNA Methylation Profiles of ERCC6 Gene in Lens Tissue from Age-Related Cortical Cataract
  Author(s): Yong Wang, Guowei Zhang, Fei Li, Lihua Kang, Jindong Ben, Han Rong, and Huaijin Guan Yong Wang, Guowei Zhang, Fei Li, Lihua Kang, Jindong Ben, Han Rong, and Huaijin Guan
  
  Ultraviolet (UV)-induced DNA damage attributes to the pathogenesis of age-related cataract (ARC) and is repaired via the nucleotide excision repair (NER). It is known that Cockayne syndrome complementation group B (CSB) protein coded by ERCC6 is a component of NER complex. DNA methylation is one of the major epigenetic events and is catalyzed by DNA 5-cytosine-methyltransferases (DNMTs). This study was to examine the potential contribution of DNA methylation of CpG islands in ERCC6 promoter region in lens tissues to ARC pathogenesis. Fifteen cortical type of ARC lenses and fifteen transparent lenses from human subjects were included in this study. ERCC6 and DNMTs expression in the lenses were analyzed by qRT-PCR and western blot. Bisulfite-sequencing PCR (BSP) was performed to evaluate methylation status of ERCC6. An in-vitro experiment by adding a demethylating agent 5-aza-2’-d.. View More»
  DOI: 10.4172/2155-9570.1000392

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