Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access

Lucy Villegas

Publications

• Research Article
  High Pregnancy and Implantation Rates Can Be Obtained Using Magnetic-Activated Cell Sorting (MACS) to Selection Spermatozoa in Patients with High Levels of Spermatic DNA Fragmentation
  Author(s): Javier García-Ferreyra, Lucy Villegas, Rocío Romero, Patricia Zavala, Roly Hilario and Julio Dueñas-Chacón Javier García-Ferreyra, Lucy Villegas, Rocío Romero, Patricia Zavala, Roly Hilario and Julio Dueñas-Chacón
  
  Purpose: DNA fragmentation in spermatozoa has been associated with poor semen quality, low fertilization rates, impaired preimplantation development, and high miscarriage rates. The objective of this study was to evaluate the effects of the use of non-apoptotic MACS-selected spermatozoa in ICSI cycles, compared to those ICSI cycles in patients with normal sperm DNA fragmentation. Methods: A total of 134 cycles of ICSI were included in the study. The study group consisted of patients with high DNA fragmentation and their spermatozoa were selected by MACS (n=57); and the control group, of patients with normal DNA fragmentation and their spermatozoa were selected by classic morphological characteristics. The fertilization rate, cleavage rate, embryo quality, pregnancy rate (PR), implantation rate (IR) and miscarriage rate (MR) were compared bet.. View More»
  DOI: 10.4172/2375-4508.1000133

  Abstract PDF

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