Fungal Genomics & Biology
Open Access
ISSN: 2165-8056
ISSN: 2165-8056
LV D
IR Iran
Research Article
The Relationship between rDNA-ITS Sequences and Biological Characteristics of the Apple Ring Rot Pathogen Botryosphaeria berengeriana de Not f. sp. piricola (Nose)
Author(s): LV D, Zhang JY, Zhang Z, Zhou ZQ, Chen XK, Du XL and Qu SC
LV D, Zhang JY, Zhang Z, Zhou ZQ, Chen XK, Du XL and Qu SC
Ten fungal strains that cause apple ring rot disease (Physalospora piricola Nose) were used to study the role of ribosomal DNA-internal transcribed spacer (rDNA-ITS) in classifying and identifying the pathogen Botryosphaeria berengeriana de Not f. sp. piricola (Nose) Koganezawa et Sakuma, and the correlation between sequence mutations and biological characteristics, and pathogenicity. We used an effective method to screen for conidiospore induction in in vitro cultures of the pathologic pathogens. PCR amplification and clone sequencing were performed for the rDNA-ITS region and used to construct a phylogenetic tree. The growth status, sporulation ability, and phylogenetic effect of different strains were determined. The influence of culture medium types, pH, temperature and light, and the effect of mycelia scraping were investigated. Based on the 582-bp and 583-bp cloned fragments, the r.. View More»
DOI:
10.4172/2165-8056.1000104