Journal of Chromatography & Separation Techniques
Open Access

M. Hegazy

Publications

• Research Article
  Simultaneous determination of Ambroxol Hydrochloride and Guaifenesin by HPLC, TLC-Spectrodensitometric and multivariate calibration methods in pure form and in Cough Cold Formulations
  
  Author(s): M. Abdelkawy, F. Metwaly, N. El Raghy, M. Hegazy and N.Fayek M. Abdelkawy, F. Metwaly, N. El Raghy, M. Hegazy and N.Fayek
  
  Three simple, reliable methods were developed for the simultaneous determination of a mixture of Ambroxol HCl (A) and Guaifenesin (G) in presence of the oxidative degradate (AD) of A and guaicol (GD), the impurity of G . The first method is an isocratic HPLC method, where separation of the four components A, AD, G and GD was achieved on C18 column using water: methanol (80: 20, v/v, containing 1% triethylamine, pH 2.9) with a flow rate of 1.5 ml/min and UV detection at 220nm. A linear relationship in the range of 5-80 μg.ml -1 and 10-150 μg.ml -1 was obtained for A and G, respectively. The second method was a TLC-spectrodensitometric method, where the drugs together with AD and GD were applied on silica gel 60F 254 plates and a mobile phase consisted of chloroform: methanol: ethyl acetate: acetic acid (70: 8: 12:10, by volume) was used for separation. Densitometric evaluation of.. View More»
  DOI: 10.4172/2157-7064.1000112

  Abstract

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