Journal of Drug Metabolism & Toxicology
Open Access

Mahima Kaushik

Publications

• Research Article
  Spectroscopic Studies of the Binding Interactions of Phenothiazininum Dyes (Thionine Acetate, Azure A and Azure B) with Calf-thymus DNA
  Author(s): Mohan Kumar, Mahima Kaushik, Swati Chaudhary and Shrikant KukretiMohan Kumar, Mahima Kaushik, Swati Chaudhary and Shrikant Kukreti
  
  The double helical structure of DNA offers various binding sites for the interaction of ligands or proteins. Interactions using minor groove, major groove, and through intercalation are the major types of binding mechanisms of DNA-ligand interactions. The lowering in the absorption intensity along with bathochromic shift is the indication of intercalation binding mode of the dye into the base pairs of the DNA. In this study, the interaction of phenothiazine dyes with calf-thymus DNA (ctDNA) in physiological buffer (pH 7.4) was studied using UV-visible, fluorescence, circular dichroism (CD), and UV-thermal denaturation spectroscopy. The binding constants were calculated at different temperatures with the help of fluorescence spectroscopy. CD signals signify that B-form of DNA might become more compact, upon binding of the dyes. Also, induced circular dichroism is observed which confirm.. View More»
  DOI: 10.4172/2157-7609.1000214

  Abstract PDF

Relevant Topics