Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access

Malgorzata Duda

Publications

• Short Communication
  Magnetic Isolation and Characterization of Porcine Ovarian Putative Stem Cells (Pscs): An In Vitro Study
  Author(s): Malgorzata DudaMalgorzata Duda
  
  According to the ruling hypothesis the potential source of tumorigenesis might be cancer stem cells (CSCs) resulting from the accumulation of mutations in other types of stem cells (SCs). Therefore we decided to identify and characterize the somatic stem cells commonly called Putative Stem Cells (PSCs) in the porcine postnatal ovary. To isolate ovarian PSCs, an antibody against SSEA-4 conjugated to magnetic beads was used. PSCs were cultured for 7 days. During the culture spontaneously arising embryoid bodies have been observed. Such behaviour under culture condition is characteristic for stem cells. Using immunofluorescence we showed cytoplasmic localization of the Oct 3/4 and Sox-2, pluripotency markers, proteins responsible for maintaining cells in an undifferentiated state. Using immunocytochemistry we showed transmembrane and cytoplasmic localization of the c-Kit receptor and GDF.. View More»
  DOI: 10.4172/2375-4508.1000191

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