Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Manoj Nath
India
Research Article
Molecular Cloning and Comparative In silico Analysis of Calmodulin Genes from Cereals and Millets for Understanding the Mechanism of Differential Calcium Accumulation
Author(s): Manoj Nath, Anshita Goel, Gohar Taj and Anil Kumar
Manoj Nath, Anshita Goel, Gohar Taj and Anil Kumar
Calmodulin gene (CaM) - a calcium sensor was cloned by the technique of polymerase chain reaction (PCR) from cereals and millets using single set of primer designed from Eleusine coracana ESTs available in dbEST database. The PCR amplicons of 613bp were consistently observed in all cereals and millets except Setaria italica. The amplicons from these cereals were further gel eluted, cloned, sequenced and subjected to homology search, multiple sequence alignment, phylogenetic tree construction and motif analysis. The homology search con fi rmed their identity to CaM genes except for Triticum aestivum. Multiple sequence alignment of the translated CaM amino acid sequences con fi rmed the presence of conserved consensus sequences of 110 amino acids, which was uniformly observed across different cereals and millets except Sorghum bicolor. Phylogenetic tree constructed base.. View More»
DOI:
10.4172/jpb.1000154