Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
+44 1223 790975
ISSN: 2155-9899
+44 1223 790975
Mansoor Amiji
School of Pharmacy,
Boston, MA 02115
Tanzania
Research Article
Macrophage Polarization and the Effect of MicroRNA-155 Administered in Water-in-Oil-in-Water Multiple Emulsion Formulations
Author(s): Adwait Oka, Meghna Talekar, Qijun Ouyang, Ed Luther and Mansoor AmijiAdwait Oka, Meghna Talekar, Qijun Ouyang, Ed Luther and Mansoor Amiji
The tumor microenvironment is composed of range of cells including macrophages that participate in sustaining tumor growth and invasion. Tumor-associated macrophages (TAM’s) polarized to the M2 or pro-tumoral phenotype are one such population of cells highly expressed in the tumor microenvironment. The expression of microRNA’s (miR’s) is often found to be dysregulated in TAM’s and hence exogenous delivery of miR provides a strategy for macrophage phenotypic modulation to attain improved anticancer activity. In this study, we assessed the use of multiple emulsions for the delivery of miR to achieve macrophage M1 repolarization. Water-in-oil-in-water (WOW) multiple emulsions (ME) were prepared using a two-step emulsification technique for encapsulation of miR-155 and cellular uptake, transfection efficiency, and repolarization capabil.. View More»
DOI:
10.4172/2155-9899.1000326