Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Manu Chaudhary
India
Research Article
Rising Antimicrobial Resistance of Pseudomonas aeruginosa Isolated from
Clinical Specimens in India
Author(s): Manu Chaudhary and Anurag Payasi
Manu Chaudhary and Anurag Payasi
The rising antibiotic resistance against commonly used drugs is of great concern. Drug susceptibility testing and Polymerase Chain Reaction (PCR) assay were used to determine the antibiotic susceptibility patterns and prevalence of genes encoding extended-spectrum-β-lactamases (ESBLs) and metallo-β-lactamases (MBLs) among 515 isolates of Pseudomonas aeruginosa isolated from various clinical specimens. Susceptibility of isolates to seven antibiotics was tested using disc diffusion method according to the guidelines defined by Clinical Laboratory Standard Institute. Isolates showing resistance to any of the two cephalosporins (ceftriaxone, ceftazidime and cefotaxime) were subjected to PCR for the prevalence of ESBL and MBL gene characterization. Out of the 515 isolates, 235 (45.63%) were considered as ESBL positive; 87 (16.89%) were MBL positi.. View More»
DOI:
10.4172/jpb.1000253