Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Maria Elizabeth P Passos
Brazil
Research Article
Signaling Pathways Involved in the Effects of Different Fatty Acids on Interleukin-2 Induced Human Lymphocyte Proliferation
Author(s): Renata Gorjão, Sandro M Hirabara, Maria F Cury-Boaventura, Thais Martins de Lima, Maria Elizabeth P Passos, Adriana C Levada-Pires and Rui CuriRenata Gorjão, Sandro M Hirabara, Maria F Cury-Boaventura, Thais Martins de Lima, Maria Elizabeth P Passos, Adriana C Levada-Pires and Rui Curi
The aim of the present study was to investigate the mechanisms involved in the modulation of human lymphocyte proliferation by different fatty acids. We evaluated the effects of palmitic (PA), stearic (SA), oleic (OA), linoleic (LA), docosahexaenoic (DHA), and eicosapentaenoic (EPA) acids on IL-2 signaling pathways in addition to the involvement of the de novo ceramide synthesis and PI3K pathway. PA, SA, DHA, and EPA decreased the JAK1/JAK3/STAT5 pathway and IL-2-induced Akt phosphorylation. OA and LA had no effect. The inhibitory effect of DHA and EPA on lymphocyte proliferation was abolished by fumonisin B1 (FB1, an inhibitor of the de novo ceramide synthesis), whereas the effect of the other fatty acids remained unchanged. ERK1/2 phosphorylation was increased by OA and LA but markedly decreased by the other fatty acids. PKC-ζ phosphorylation was increased by OA and LA.. View More»
DOI:
10.4172/2155-9899.1000171