Cell & Developmental Biology
Open Access
ISSN: 2168-9296
+44 1478 350008
ISSN: 2168-9296
+44 1478 350008
Research Article
Characterization of Cell Cycle Phase-Based Micrornas in Pluripotency and Differentiation
Author(s): Xiaoyan Ming, Timothy Ming-Hun Wan, Lin Chen, Maria Mercedes Garcia-Barcelo1 and Chung Mau Lo, Xiao Qi WangXiaoyan Ming, Timothy Ming-Hun Wan, Lin Chen, Maria Mercedes Garcia-Barcelo1 and Chung Mau Lo, Xiao Qi Wang
In addition to signaling pathways, transcription factors and epigenetic regulators, microRNAs (miRNAs) are emerging as important regulators of human pluripotent stem cells (hPSCs). Pluripotent miRNAs that regulate G1-S transition and pluripotency factors to maintain self-renewal have been identified. However, only 4-5 clusters of miRNAs have been identified in human embryonic stem cells (hESCs). We performed cell cycle phase-based (G1, S, and G2/M phases) miRNA array in pluripotent and differentiated hESCs. We demonstrated that embryonic stem cell-cell cycle (ESCC) regulating miRNAs were all highly expressed in three cell cycle phases of undifferentiated hESCs suggesting a non-cell phase regulated mechanism. From cell phase-dependent miRNAs, G2/M-miRNAs was extracted by principle component analysis (PCA) as a significant component in pluripotent hESCs, whereas G1-miRNAs was a signific.. View More»
DOI:
10.4172/2168-9296.1000178