Journal of Chromatography & Separation Techniques
Open Access

Mario Marini

Publications

• Research Article
  Substance P Self-Aggregation Revised: A Chromatographic and Mass Spectrometry Analysis
  Author(s): Chiara Bignardi, Antonella Cavazza, Mario Marini and Giorgio Roda L Chiara Bignardi, Antonella Cavazza, Mario Marini and Giorgio Roda L
  
  Size exclusion liquid chromatography and electrospray ionization mass spectrometry have been used for the first time to analyze the possible effect of the environmental conditions on substance P (Arg-Pro-Lys-Pro-Gln-Gln- Phe-Phe-Gly-Leu-Met-NH2) self-association. The effects of different parameters such as solvent ionic strength, pH and polarity have been evaluated by monitoring the changes observed in aggregation forms registered by size exclusion chromatography. Mass spectrometry has been employed with the aim of identifying the size of the aggregation clusters formed in gaseous state. These two techniques can be considered complementary since they allow describing the same phenomena by different points of view permitting to identify the possible role of environmental parameters in controlling the distribution of different sized aggregates. Thus, new details can be added to the prev.. View More»
  DOI: 10.4172/2157-7064.1000140

  Abstract PDF

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