Journal of Clinical & Experimental Dermatology Research
Open Access

Marléne Isaksson

Publications

• Research Article
  Factors Affecting the Concentration of Diphenylmethane-4,4′-Diisocyanate in Freund’s Complete Adjuvant. Can They Affect the Outcome of the Guinea-Pig Maximization Test?
  Author(s): Haneen Hamada, Magnus Bruze, Erik Zimerson, Marléne Isaksson, Malin EngfeldtHaneen Hamada, Magnus Bruze, Erik Zimerson, Marléne Isaksson, Malin Engfeldt
  
  Background: The animal assay Guinea Pig Maximization Test (GPMT) uses Freund’s complete adjuvant (FCA) to enhance the dermal sensitization in the animals. In a previous study, the degree of sensitization when investigating diphenylmethane-4,4′-diisocyanate (4,4′-MDI) varied. 4,4′-MDI is known to be a reactive diisocyanate and concern was risen that its instability and reaction with FCA might affect the outcome. Objectives: To investigate the stability of 4,4′-MDI and its possible reaction with components in FCA. Methods: The same preparations as used in the GPMT were prepared, i.e. 1.0% w/v 4,4ʹ-MDI in FCA/paraffin oil (40/60 v/v) and stored under different conditions for 48 hours. The content of 4,4′-MDI in both FCA/paraffin oil mixtures as well as in samples of pure substance were.. View More»
  DOI: 10.4172/2155-9554.1000402

  Abstract PDF

Relevant Topics