Journal of Proteomics & Bioinformatics
Open Access

Masayo Yamada

Publications

• Research Article
  The Proteomic Profile of Pancreatic Cancer Cell Lines Corresponding to Carcinogenesis and Metastasis
  Author(s): Masayo Yamada, Kiyonaga Fujii, Koji Koyama, Setsuo Hirohashi and Tadashi Kondo Masayo Yamada, Kiyonaga Fujii, Koji Koyama, Setsuo Hirohashi and Tadashi Kondo
  
  To investigate the proteomic background of the carcinogenesis and progression of pancreatic cancer, the protein expression profiles of nine well-characterized pancreatic adenocarcinoma cell lines, whose metastatic potential was previously examined in a mouse xenograft model, and two immortalized pancreatic duct cell lines were examined. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 126 protein spots the intensity of which was significantly different between the normal pancreatic duct cell lines and the pancreatic cancer cell lines with different metastatic potential. Mass spectrometric protein identification demonstrated that these protein spots corresponded to 95 unique genes, which included proteins not previously shown to be aberrant in pancreatic cancer. To characterize the observed proteome, LC-MS/MS identified the proteins corresponding to .. View More»
  DOI: 10.4172/jpb.1000057

  Abstract PDF

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