Journal of Proteomics & Bioinformatics
Open Access

Matthew Kuruc

Publications

• Short Communication
  AlbuVoid™ Coupled to On-Bead Digestion - Tackling the Challenges of Serum Proteomics
  Author(s): Haiyan Zheng, Caifeng Zhao, Meiqian Qian, Swapan Roy, Absari Arpa, Amenah Soherwardy and Matthew KurucHaiyan Zheng, Caifeng Zhao, Meiqian Qian, Swapan Roy, Absari Arpa, Amenah Soherwardy and Matthew Kuruc
  
  For cancer research, serum and plasma are especially attractive sample types as collection of blood is common, simple and only minimally invasive. Yet serum samples can offer unique challenges in LC-MS proteomic analyses. The two biggest challenges being: 1) the high abundance of Albumin accounting for about 50% of the total protein mass and, 2) proteolytic resistance, in large part due to substantial amount of glycoprotein, a modification that manifests proteolytic resistance. In this short report, we describe new methods using a surface/bead based product, AlbuVoid™, which depletes Albumin through a negative selection or voidance strategy, retaining the vast amount of the remaining serum proteome on the bead. We then combine this novel enrichment, with a direct and seamless integration with Trypsin digestion, a method conventionally referred to as on-bead digestion. We evaluat.. View More»
  DOI: 10.4172/jpb.1000373

  Abstract PDF

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