Medicinal & Aromatic Plants
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Mbamalu ON

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• Research Article
  HPLC Determination of Selected Flavonoid Glycosides and their Corresponding Aglycones in Sutherlandia frutescens Materials
  Author(s): Mbamalu ON, Antunes E, Silosini N, Samsodien H and Syce J Mbamalu ON, Antunes E, Silosini N, Samsodien H and Syce J
  
  Sutherlandia frutescens is a popular South African plant commercially available in a range of formulations. However, reference standards for quality and stability assessment are lacking. This work reports the development and validation of a reversed phase HPLC method for the analysis of flavonoid glycosides and their corresponding aglycones in S. frutescens products. Five materials containing either leaf powder (LP) or spray-dried aqueous extract (SDAE) of S. frutescens were analysed for flavonoid content. A primary objective was to isolate non-commercially available flavonoid glycoside compounds (sutherlandins) for use as reference standards. Sutherlandins A, B, C and D were successfully isolated, and used, with other flavonoid compounds for HPLC assay development. The developed HPLC method was linear in the range of 0.2 to 60 μg/ml for quercitrin; 0.2 to 120 μg/ml for querceti.. View More»
  DOI: 10.4172/2167-0412.1000246

  Abstract PDF

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