Journal of Clinical & Experimental Dermatology Research
Open Access

Meera Brahmbhatt

Publications

• Research Article
  Proliferative Nodules in Congenital Nevi - A Histopathologic, Genetic and Immunohistochemical Reappraisal
  Author(s): Meera Brahmbhatt, Shi Yang and Meera Mahalingam Meera Brahmbhatt, Shi Yang and Meera Mahalingam
  
  Although histopathology is the gold standard for the classification of melanocytic proliferations, in some such as the proliferative nodule, an equivocal diagnosis can be diagnostically extremely challenging based on histopathologic features alone. The purpose of this study was to review the histologic features of proliferative nodules and to ascertain the utility of immunohistochemistry and analyses of oncogenic mutations in signaling components of the MAP kinase pathway as diagnostic adjuncts. Genomic DNA for genotyping was isolated per protocol using techniques that included laser capture microdissection to isolate nevus cells from proliferative nodules (n=3) and age-matched congenital nevi (CN, n=3). Direct DNA sequencing was performed on BRAF codon 600; NRAS1 codons 12 and 13; NRAS2 codons 60 and 61, KRAS codons 12 and 13 and GNAQ. Immunohistoc.. View More»
  DOI: 10.4172/2155-9554.1000105

  Abstract PDF

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