Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Michael Kohl
Medizinisches Proteom-Center, ZKF E.2.051, Ruhr- Universitaet Bochum, Bochum, Universitaetsstr. 150,
D-4801 Bochum
Germany
Research Article
Automated Calculation of Unique Peptide Sequences for Unambiguous Identification of Highly Homologous Proteins by Mass Spectrometry
Author(s): Michael Kohl, Gorden Redlich, Martin Eisenacher, Anke Schnabel, Helmut E. Meyer, Katrin Marcus and Christian StephanMichael Kohl, Gorden Redlich, Martin Eisenacher, Anke Schnabel, Helmut E. Meyer, Katrin Marcus and Christian Stephan
In regular proteomics approaches proteases are used to digest the proteome into a set of peptides. Unfortunately, determining proteins on the basis of peptides implies some uncertainty since a peptide may be part of different proteins. Therefore, a targeted detection of unique peptides of particular proteins is a promising task for an unambiguous identification of a specific protein. Here we present a software solution that offers the possibility for a highly efficient and simple detection of such unique peptides. In a first step a SQL-based database of theoretically digested peptides from a given FASTA file formatted protein database is generated by choosing a protease. In a second step, in silico generated peptides from a pre-defined protein sequence are compared to this peptide database in order to identify unique peptides. Amongst others, possible.. View More»
DOI:
10.4172/jpb.1000003