Journal of Proteomics & Bioinformatics
Open Access

Michael V Filatov

Publications

• Research Article
  Proteomic Profiling of High-grade Glioblastoma Using Virtual experimental 2DE
  Author(s): Stanislav N Naryzhny, Maria A Maynskova, Victor G Zgoda, Natalia L Ronzhina, Svetlana E Novikova, Natalia V Belyakova, Olga A Kleyst, Olga K Legina, Rimma A Pantina and Michael V Filatov Stanislav N Naryzhny, Maria A Maynskova, Victor G Zgoda, Natalia L Ronzhina, Svetlana E Novikova, Natalia V Belyakova, Olga A Kleyst, Olga K Legina, Rimma A Pantina and Michael V Filatov
  
  Identification and quantitative analysis of different proteoforms (protein species) presented in a cell line generated from high grade glioblastoma was performed using two-dimensional electrophoresis (2DE), mass spectrometry (ESI LC-MS/MS), and immunodetection. A 2DE protein map containing an extensive data set comprising 937 spots with 1542 unique protein identifications (proteoforms) of 600 genes was obtained. Additionally, another set of experiments was performed where 16012 proteoforms coded by 4050 genes were identified by MS/MS according to their position in 96 gel sections (pixels). A special attention has been paid to the proteins that are the potential biomarkers of glioblastoma. The list of these biomarkers was compiled from literature. Next, we generated the graphs with theoretical and experimental information about proteoforms coded by the same gene. Such a virtualexperime.. View More»
  DOI: 10.4172/jpb.1000402

  Abstract PDF

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