Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
+44 1478 350008
ISSN: 2161-0495
+44 1478 350008
Mikio Marumo
Department of Environmental and Preventive Medicine, Hyogo College of Medicine, Mukogawa-cho 1-1, Nishinomiya, Hyogo 663-8501,
Japan
Research Article
Ethanol-Induced Inhibition of Platelet Aggregation in Whole Blood from Healthy Donors
Author(s): Mikio Marumo and Ichiro Wakabayashi
Mikio Marumo and Ichiro Wakabayashi
Ethanol is known to inhibit platelet aggregation. In order to examine effects of ethanol on platelet aggregation, isolated platelet-rich plasma and platelet suspension are often used. However, it remains to be clarified whether and how ethanol affects platelet aggregation in whole blood. In this concise study, we examined effects of ethanol on platelet aggregation induced by different stimulants in whole blood by using the screen filtration pressure method. Thapsigargin and 1-oleoyl-2-acetyl-sn-glycerol (OAG), which cause Ca2+ entry into platelets through the storeoperated Ca2+ channels and the diacylglycerol-dependent Ca2+ channels, respectively, were used for inducing platelet aggregation. Thapsigargin-induced platelet aggregation was markedly inhibited by ethanol at 0.25% or higher, and OAG-induced platelet aggregation was significantly inhibited by ethanol at 1% or higher. These r.. View More»
DOI:
10.4172/2161-0495.1000239