Journal of Clinical Chemistry and Laboratory Medicine
Open Access
ISSN: 2736-6588
ISSN: 2736-6588
Miklos Oldal
Hungary
Research Article
Droplet Digital PCR Analysis of miR-191-3p in the Spent Blastocyst Culture Media Might Reflect the Reproductive Competence of the 3rd Day Human Embryo
Author(s): Katalin Gombos, Miklos Oldal, Kristztina Ildiko Kalacs, Kristztina Godony, Akos Varnagy, Jozsef Bodis and Gabor L KovacsKatalin Gombos, Miklos Oldal, Kristztina Ildiko Kalacs, Kristztina Godony, Akos Varnagy, Jozsef Bodis and Gabor L Kovacs
Background: Droplet digital PCR analysis of human embryonic culture media collected in a proof of con-cept study on IVF pregnancy outcome. miR-191-3p was analysed in spent human embryonic culture media of morphologically similar, good quality embryos which after later transfer developed either to reproductively competent embryos and healthy neonates or of those, where miscarriage occurred in early stage of the pregnancy. Methods: Spent culture media at the morula-blastocyst stage (3rd day) were collected from embryos fertilised with ICSI and undergoing embryo transfer. After registered pregnancy outcome 40 samples from the group of reproductively competent embryos, 40 samples from miscarriage and their parallel blank culture media samples were enrolled in miRNA analysis. Isolation and quantitative detection of miRNA from embryonic culture media was carried out on automated droplet dig.. View More»