Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Mir Yasir Arfat
India
Research Article
Relevance of Nitroxidation of Albumin in Rheumatoid Arthritis: A Biochemical and Clinical Study
Author(s): Zarina Arif, Mir Yasir Arfat, Jamal Ahmad, Asif Zaman, Shireen Naaz Islam and M Asad KhanZarina Arif, Mir Yasir Arfat, Jamal Ahmad, Asif Zaman, Shireen Naaz Islam and M Asad Khan
Objective: To study the role of peroxynitrite-modified human serum albumin (nitroxidized-albumin) in rheumatoid arthritis. Methods: Human serum albumin was exposed to peroxynitrite and changes in albumin structure were monitored by UV-visible, fluorescence and circular dichroism spectroscopy, thioflavin T, Congo red binding and attenuated total reflection-Fourier transformed infrared spectroscopy (ATR-FTIR). Antioxidant properties of nitroxidized-albumin were evaluated by free radical induced RBC hemolysis test. Markers of protein oxidation like carbonyl, thiol, dityrosine and RBC hemolysis were evaluated in RA patients’ sera. Binding of autoantibodies in RA sera (n=50) with nitroxidized-albumin was studied by direct binding, inhibition ELISA and electrophoretic mobility shift gel assay. Results: The nitroxidized-albumin indicated the generation of n.. View More»
DOI:
10.4172/2155-9899.1000324