Journal of Proteomics & Bioinformatics
Open Access

Mohammad Hossein Ghahremani

Publications

• Short Communication
  Design of in silico Trifunctional Antibody (hIgG1-FC: mouse-antiHER2? human-B7.1) Gene Cassettes and Expression Vectors: The Stage Prior to Production
  Author(s): Mohammad Reza Dehghani, Mohammad Hossein Ghahremani and Kobra OmidfarMohammad Reza Dehghani, Mohammad Hossein Ghahremani and Kobra Omidfar
  
  The specific gene cassettes and expression vectors for tri-functional antibody (hIgG1-FC: mouse-antiHER2× human-B7.1) were designed in silico. The in silico tri-functional antibody has been derived from human IgG1, human B7.1, and mouse antiHER2 antibody. The variable domains of IgG1 framework were substituted by Cha21 (mouseantiHER2 scFV) VH-VL, and B7.1 IgV-like domains. The domain data of human IgG1 heavy and light chain-constant regions, human B7.1 IgV-like, and cha21 variable regions were obtained from UniprtoKB and PDB databases. The 3D structure of domains was trimmed and modeled by pyMol software. To ensure the presence of antigen binding residues, complementarity determining regions, and framework regions in variable domain structure, the researchers analyzed the cha21-VH and VL nucleotide sequences using Paratome and IMGT softwares, respectively. In this study, two in s.. View More»
  DOI: 10.4172/jpb.1000339

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