Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Mohanan PV
Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology,
Thiruvananthapuram, Kerala
India
Research Article
Protective Mechanism of Melatonin on Kainic Acid Induced Immune Modulatory Effect on Lymphocytes Derived from Mouse Spleen
Author(s): Gayathri V and Mohanan PVGayathri V and Mohanan PV
Toxicity occurs when cells develop inflammation due to exposure to several toxic substances. The study was aimed to assess the damage (toxicity) induced by kainic acid on splenic lymphocyte and enriched T lymphocyte under in vitro conditions and the protective role of exogenous melatonin against this damage (toxicity). The study included assessment of inflammatory mechanism by the expression of immune modulatory cytokine mediators using real time PCR. Oxidative stress (reactive oxygen species) and nitrosative stress (reactive nitrogen species) were also studied to determine the free radical production. Interestingly, kainic acid caused severe splenic lymphocytes and enriched T lymphocyte damage which was evident from deleterious alterations in various parameters. Kainic acid treatment (1 mM) resulted in increased mRNA expression of cytokines like tumour necrosis factor-beta, .. View More»
DOI:
10.4172/2155-9899.1000172